VISTA inhibitor III |CAS:2705841-87-6 Probechem Biochemicals

Chemical Structure : VISTA inhibitor III

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VISTA inhibitor III

货号: PC-38146Not For Human Use, Lab Use Only.

VISTA inhibitor III is a small-molecule ligand for immune checkpoint VISTA with IC50 of 480 nM in FRET assay.

规格	价格	库存	数量
25 mg		Get quote
50 mg		Get quote
100 mg		Get quote

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生物&药学活性

VISTA inhibitor III is a small-molecule ligand for immune checkpoint VISTA with IC50 of 480 nM in FRET assay.
The binding affinity of VISTA inhibitor III to VISTA was confirmed by competitive ELISA with IC50 value of 716 nM, Kd of 247 nM in SPR assay.
VISTA inhibitor III blocks VISTA-VISTA interaction resulting in decreased IL-2 signaling in Promega VISTA bioluminescent cell-based T-cell activation assay.
Similarly, to VISTA antibody, addition of VISTA inhibitor III to co-cultured Jurkat T cells with 8 cancer cell lines selectively restored the proliferation of T cells in the presence of high VISTA expressing cell lines (OVKATE, SKOV3, COV504, RL952, HEC1A) while minimal effect was evident for cancer cell lines with low VISTA expression (A2780, AN3CA, Ishikawa).
VISTA inhibitor III also upregulated interferon gamma (IFNγ) and tumor necrosis factor-alpha (TNFα), cytokines secreted by activated T cells in in the supernatants of co-cultures of purified human T cells with OVKATE and RL952 cell lines with high VISTA expression.

物理化学性质&存储条件

分子量	393.523
分子式	C21H19N3OS2
外观性状	Solid
CAS No.
储存条件	固体粉末 -20°C 12 个月; 4°C 6 个月	配置液 -80°C 6 个月; -20°C 6 个月
Shipping
Solubility	10 mM in DMSO
Chemical Name/SMILES	(Z)-N-ethyl-N-phenyl-3-(((5-phenylfuran-2-yl)methyl)imino)-3H-1,2,4-dithiazol-5-amine

参考文献

1. Moustafa T Gabr, et al. J Am Chem Soc. 2020 Sep 23;142(38):16194-16198.